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Roles of transient receptor potential channels in eclosion and movement in the red flour beetle Tribolium castaneum
Author(s) -
Kim Hong Geun,
Margolies David C.,
Park Yoonseong
Publication year - 2018
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/phen.12232
Subject(s) - biology , tonic (physiology) , red flour beetle , drosophila melanogaster , transient receptor potential channel , receptor , microbiology and biotechnology , anatomy , endocrinology , genetics , insect , botany , gene
Transient receptor potential channels (TRPs) are a family of cation channels involved in various sensory mechanisms in Drosophila melanogaster , including mechanosensing. Phylogenetic analysis of mechanosensory TRPs in seven members of the TRPV, TRPN and TRPA subfamilies reveals a unique TcTrpA5 in Tribolium castaneum that is likely lost in D. melanogaster . Because mechanosensors are implicated in various key physiology, we investigate the roles of eight candidate mechanosensory TRPs in survival, walking behaviour and tonic immobility in T. castaneum using the RNA interference technique. Double‐stranded RNA treatment of nompC (ds NompC ) and trpA5 (ds TrpA5 ) results in eclosion failure, causing 93% mortality of beetles subjected to each of these treatments. The beetles that survive the ds NompC treatment show defects during sclerotization of the elytra. Adult beetles treated with ds Nanchung and ds Inactive show defects in the folding of the joint between the femur and tibia segments of the hind legs, resulting in abnormal walking behaviour and reduced walking speed. Regarding tonic immobility induced by mechanical stimulations on the ventral surface, knockout responses (death feignings) are significantly extended with ds Nanchung , ds Inactive and ds Waterwitch treatments. These data suggest that both nompC and trpA5 are required for adult eclosion. The functions of nanchung and inactive are involved in the folding the hind leg segments. In addition, nanchung , inactive , and waterwitch are likely the receptors involved in recovery from tonic immobility.