Premium
Comparative transcriptomics support evolutionary convergence of diapause responses across I nsecta
Author(s) -
Ragland Gregory J.,
Keep Evan
Publication year - 2017
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/phen.12193
Subject(s) - diapause , biology , transcriptome , insect , phylogenetic tree , evolutionary biology , gene , genetics , zoology , larva , gene expression , ecology
Diapause is a common phenotype that is broadly phylogenetically dispersed across I nsecta and appears to have multiple evolutionary origins. Nevertheless, there are clear commonalities in diapause regulation across insect taxa. In the present study, we report a meta‐analysis of diapause whole transcriptomic data sets from 11 different insect species that addresses three questions: (i) how similar are whole‐transcriptome diapause responses across species within and across different diapause life‐cycle stages; (ii) do the most closely‐related species demonstrate the most similar diapause responses within and across diapause life‐cycle stages (the existence of phylogenetic signal); and (iii) is there a core set of regulatory genes that universally associate with insect diapause at the transcript level? The included species are mostly D ipterans ( n = 9), plus one species each from L epidoptera and H ymenoptera. The group includes multiple species that enter diapause as either larvae, pupae or adults. We establish a set of 4791 orthologous transcript sequences with expression data acquired from published studies of diapause transcriptomes. We find no support for phylogenetic signal. Transcriptomic responses of non d ipterans clustered within rather than outside of D ipteran responses. However, expression profiles do tend to cluster with the diapause stage of developmental arrest, although this pattern is only moderately supported. We identify a statistically significant set of 542 orthologues (11% of all orthologues) that are commonly differentially regulated during diapause across all included species. From this core set, we identify candidate genes participating in circadian rhythmicity, insulin signalling and W nt signalling, which are pathways previously associated with insect diapause development. Clustering relationships among species are most consistent with evolutionary convergence of the shared transcriptomic response, although we are unable to determine whether this reflects convergence of the diapause initiation, maintenance or termination phases.