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S6 protein kinase activates Juvenile Hormone and vitellogenin production in the cockroach B lattella germanica
Author(s) -
Abrisqueta Marc,
SürenCastillo Songül,
Maestro José L.
Publication year - 2017
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/phen.12156
Subject(s) - biology , p70 s6 kinase 1 , vitellogenin , ribosomal protein s6 , cockroach , juvenile hormone , gene isoform , gene knockdown , phosphorylation , protein kinase a , messenger rna , kinase , medicine , microbiology and biotechnology , endocrinology , biochemistry , hormone , protein kinase b , gene , ecology
Nutritional status regulates different processes, such as growth and development, through TOR (target of rapamycin) and insulin receptor signalling pathways. The ribosomal S6 protein kinase ( S6K ) is a downstream element of both pathways. Using cDNA from the G erman cockroach B lattella germanica ( L .), two S6K isoform sequences ( BgS6K ) are cloned. The long isoform differs from the short one by the insertion of a 22‐amino acid duplication, involving the key phosphorylation position T hr 390 . As a result of this, the long isoform presents a new, potentially regulatory phosphorylation site. RNA interference knockdown of both BgS6K isoforms induces an increase in the length of the last nymphal instar, together with a reduction in the mRNA levels of a number of enzymes of the Juvenile Hormone biosynthetic pathway in the corpora allata, vitellogenin mRNA levels in the fat body and basal oocyte length. Thus, BgS6K is important for nymphal development and is necessary for the full induction of Juvenile Hormone synthesis and vitellogenin production in adult females.