Premium
Mass cytometry reveals a distinct immunoprofile of operational tolerance in pediatric liver transplantation
Author(s) -
Lau Audrey H.,
Vitalone Matthew J.,
Haas Kelly,
Shawler Todd,
Esquivel Carlos O.,
Berquist William E.,
Martinez Olivia M.,
Castillo Ricardo O.,
Krams Sheri M.
Publication year - 2016
Publication title -
pediatric transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.457
H-Index - 69
eISSN - 1399-3046
pISSN - 1397-3142
DOI - 10.1111/petr.12795
Subject(s) - flow cytometry , medicine , mass cytometry , cytometry , immunology , immune system , il 2 receptor , population , transplantation , cd38 , immune tolerance , t cell , phenotype , biology , stem cell , microbiology and biotechnology , biochemistry , environmental health , cd34 , gene
Long‐term IS in transplant patients has significant morbidity, poorer quality of life, and substantial economic costs. TOL, defined as graft acceptance without functional impairment in the absence of IS, has been achieved in some pediatric LT recipients. Using mass cytometry, peripheral blood immunotyping was performed to characterize differences between tolerant patients and patients who are stable on single‐agent IS. Single‐cell mass cytometry was performed using blood samples from a single‐center pediatric LT population of operationally tolerant patients to comprehensively characterize the immune cell populations in the tolerant state compared with patients on chronic low‐dose IS. Specific T‐cell populations of interest were confirmed by flow cytometry. This high‐dimensional phenotypic analysis revealed distinct immunoprofiles between transplant populations as well as a CD 4 + TOT ( CD 4 + CD 5 + CD 25 + CD 38 −/lo CD 45 RA ) that correlates with tolerance in pediatric LT recipients. In TOL patients, the TOT was significantly increased as compared to patients stable on low levels of IS. This TOT cell was confirmed by flow cytometry and is distinct from classic Treg cells. These results demonstrate the power of mass cytometry to discover significant immune cell signatures that have diagnostic potential.