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Neutrophil function in children following allogeneic hematopoietic stem cell transplant
Author(s) -
Kent Michael W.,
Kelher Marguerite R.,
Silliman Christopher C.,
Quis Ralph
Publication year - 2016
Publication title -
pediatric transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.457
H-Index - 69
eISSN - 1399-3046
pISSN - 1397-3142
DOI - 10.1111/petr.12714
Subject(s) - medicine , cord blood , elastase , bone marrow , progenitor cell , stem cell , phagocytosis , regimen , haematopoiesis , hematopoietic stem cell transplantation , immunology , degranulation , transplantation , gastroenterology , biology , biochemistry , genetics , enzyme , receptor
HSCT is a lifesaving procedure for children with malignant and non‐malignant conditions. The conditioning regimen renders the patient severely immunocompromised and recovery starts with neutrophil ( PMN ) engraftment. We hypothesize that children demonstrate minimal PMN dysfunction at engraftment and beyond, which is influenced by the stem cell source and the conditioning regimen. Peripheral blood was serially collected from children at 1 to 12 months following allogeneic HSCT . PMN superoxide ( O 2 − ) production, degranulation (elastase), CD 11b surface expression, and phagocytosis were assessed. Twenty‐five patients, mean age of 10.5 yr with 65% males, comprised the study and transplant types included: 14 unrelated cord blood stem cells (cords), seven matched related bone marrow donors, three matched unrelated bone marrow donors, and one peripheral blood progenitor cells. Engraftment occurred at 24 days. There were no significant differences between controls and patients in PMNO 2 − production, phagocytosis, CD 11b surface expression, and total PMN elastase. Elastase release was significantly decreased <6 months vs. controls (p < 0.05) and showed normalization by six months for cords only. The conditioning regimen did not affect PMN function. PMN function returns with engraftment, save elastase release, which occurs later related to the graft source utilized, and its clinical significance is unknown.