Premium
The hematopoietic stem cell number in the peripheral blood of pediatric recipients correlates with the outcome after living donor liver transplantation
Author(s) -
Gautier Sergey V.,
Shevchenko Olga P.,
Tsirulnikova Olga M.,
Kurabekova Rivada M.,
Lugovskaya Svetlana A.,
Naumova Elena V.,
Tsirulnikova Irina E.,
Dolgov Vladimir V.
Publication year - 2015
Publication title -
pediatric transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.457
H-Index - 69
eISSN - 1399-3046
pISSN - 1397-3142
DOI - 10.1111/petr.12482
Subject(s) - medicine , cd34 , liver transplantation , flow cytometry , haematopoiesis , immune system , hematopoietic stem cell transplantation , gastroenterology , stem cell , transplantation , hematopoietic stem cell , liver function , hematopoietic cell , immunology , cell , genetics , biology
It has been proposed that circulating HSC s play a role in graft survival after liver transplantation. The aim was to analyze the relationship between the number of HSC s before and after LDLT and liver function, immune biomarkers, and clinical outcomes in pediatric patients. We studied 15 pairs of adult healthy liver donors and pediatric recipients with ESLD . The CD 34/ CD 45+ cell number was measured in the blood via flow cytometry, and plasma levels of immune biomarkers – via ELISA . CD 34/ CD 45+ cell number in the recipients decreased within the first week after LDLT . The cell number before LDLT was negatively correlated with the plasma levels of CRP and the development of graft dysfunction in the early post‐transplant period. After LDLT , the CD 34/ CD 45+ cell number was positively correlated with the pretransplant plasma level of sCD 40L, a T‐cell activation marker. In adult liver donors, the cell number did not change within the first week after liver resection and was lower than in pediatric recipients. The results suggest that in pediatric recipients, the HSC number may be associated with graft function and could be regarded as a potential predictor of the clinical outcome after LDLT .