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MRD detection of leukemia relapse using HLA typing by FACS in combination with FISH after mismatched allogeneic stem cell transplantation
Author(s) -
Miyachi Mitsuru,
Watanabe Eri,
Watanabe Nobukazu,
Tsuma Yusuke,
KawashimaGoto Sachiko,
Tamura Shinichi,
Imamura Toshihiko,
Ishida Hiroyuki,
Hosoi Hajime
Publication year - 2014
Publication title -
pediatric transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.457
H-Index - 69
eISSN - 1399-3046
pISSN - 1397-3142
DOI - 10.1111/petr.12267
Subject(s) - human leukocyte antigen , flow cytometry , medicine , transplantation , typing , leukemia , immunology , hematopoietic stem cell transplantation , allele , fish <actinopterygii> , cancer research , microbiology and biotechnology , biology , antigen , gene , genetics , fishery
Loss of mismatched HLA is a cause of relapse following HLA ‐mismatched allo‐ SCT . We directly detected the loss of mismatched HLA alleles in relapsed leukemic cells at a MRD level using HLA typing by multicolor FACS ( HLA ‐Flow) in combination with FISH in the BM of two patients with MLL ‐ AF 9‐ positive AML , at 6 and 10 months after mismatched allo‐ SCT . HLA ‐Flow with FISH analysis detected relapsed leukemic cells not expressing a mismatched HLA allele and harboring the MLL rearrangement. Simultaneously, real‐time quantitative RT ‐ PCR detected a low copy number of MLL ‐ AF 9 transcripts, consistent with MRD detection. HLA ‐Flow with FISH is a powerful method for detecting molecular relapse after mismatched allo‐ SCT and provides important information on the HLA expression status of the relapsed leukemic cells to help determine the next intervention.