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Vitamin D supplementation does not improve plasma thiol/disulfide homeostasis
Author(s) -
Mertoglu Cuma,
Siranli Gulsah,
Topal Ismail,
Gok Gamze,
Erel Ozcan
Publication year - 2018
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/ped.13705
Subject(s) - thiol , homeostasis , medicine , endocrinology , pathophysiology , disulfide bond , vitamin , vitamin d and neurology , vitamin c , biochemistry , chemistry
Background Impairment of thiol/disulfide homeostasis, as well as vitamin D deficiency, are responsible for the pathophysiology of many acute and chronic diseases. This study examined the relationship between thiol/disulfide homeostasis and vitamin D level and supplementation. Methods A total of 203 healthy children were included in the study. The participants were divided into four groups according to 25‐hydroxyvitamin D (25( OH )D) level: group 1, severe deficiency (<10 ng/ mL ); group 2, deficiency (10–20 ng/ mL ); group 3, insufficiency (20–30 ng/ mL ); and group 4, sufficiency (≥30 ng/ mL ). Furthermore, group 5 was defined as being on vitamin D supplementation. Result Native thiol was lower in group 5 than in groups 2–4 ( P = 0.003). Disulfide was higher in groups 1, 4 and 5 than groups 2 and 3 ( P < 0.001). Total thiol was lower in group 5 than in group 4 ( P = 0.032). The ratio of native thiol/total thiol was lower in groups 1 and 5 compared with groups 2 and 3, and in group 4 compared with group 3 ( P < 0.001). The ratios of disulfide/total thiol and disulfide/native thiol were higher in groups 1 and 5 than in groups 2 and 3 whereas only the disulfide/total thiol ratio was higher in group 4 than in group 3 ( P < 0.001). Conclusions In healthy children, severe deficiency of vitamin D causes impairment of thiol/disulfide homeostasis and increases protein oxidation, which cannot be reversed by external vitamin D supplementation.