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Immunochromatography test for rapid diagnosis of Mycoplasma pneumoniae infection
Author(s) -
Onari Shigeru,
Okada Takashige,
Okada Takafumi,
Okano Syuko,
Kakuta Osamu,
Kutsuma Hirokazu,
Kobayashi Masaaki,
Kondo Yasuo,
Sakaguchi Naoya,
Tajima Takeshi,
Nagao Masayoshi,
Nakayama Eiichi,
Niimi Ryo,
Nishimura Syuichi,
Higashidate Yoshihito,
Hikita Toshiyuki,
Meguro Hidenori,
Mori Toshihiko,
Yoto Yuko,
Tsutsumi Hiroyuki
Publication year - 2017
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/ped.13383
Subject(s) - mycoplasma pneumoniae , medicine , loop mediated isothermal amplification , gold standard (test) , direct agglutination test , antigen , antibody , latex fixation test , microbiology and biotechnology , virology , immunology , pneumonia , serology , dna , biology , genetics
The sensitivity and specificity of a new rapid Mycoplasma pneumoniae antigen immunochromatography ( IC ) test, DK ‐ MP ‐001, were determined using particle agglutination ( PA ) antibody response and loop‐mediated isothermal amplification ( LAMP ) gene detection as the gold standard. Of 165 patients, 59 were diagnosed with M. pneumoniae infection based on a ≥fourfold rise of serum PA antibody during the course of the illness. Of the first visit swabs, 60 were positive for M. pneumoniae on LAMP , and 49 were positive for M. pneumoniae antigen on IC test. Compared with PA antibody and LAMP , the sensitivity/specificity of the IC test were 81.4% (48/59) and 99.1% (105/106); and 81.7% (49/60) and 100% (105/105), respectively. IC test detected antigen in pharyngeal swabs more sensitively than in nasal swabs for the same subjects ( P < 0.05). The IC test performs well enough to be used with pharyngeal swabs at the first examination.