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Rapid urine antibody test for Helicobacter pylori infection in adolescents
Author(s) -
Okuda Masumi,
Mabe Katsuhiro,
Lin Yingsong,
Chaochen Wang,
Taniguchi Yohei,
Kato Mototsugu,
Kikuchi Shogo
Publication year - 2017
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/ped.13286
Subject(s) - medicine , urine , helicobacter pylori , gastroenterology , antibody , concordance , urinalysis , population , spirillaceae , predictive value , immunology , gastritis , environmental health
Background Helicobacter pylori infection is associated with gastric cancer; thus, early diagnosis and treatment are crucial. Given that H. pylori infection in adolescents or young adults has few symptoms, screening tests are necessary for this population. In this study, the accuracy of the rapid urine H. pylori antibody (u‐HpAb) test was evaluated and compared with that of urine and serum H. pylori enzyme‐linked immunosorbent assay (u‐Hp ELISA and s‐Hp ELISA , respectively) in junior high school students. Methods All 1,225 students attending the junior high schools in Sasayama City were invited to participate in this study. Urine and blood samples were assayed for anti‐ H. pylori immunoglobulin G antibodies, and rapid u‐HpAb was performed by three investigators independently. When all investigators were in agreement, the test was confirmed as positive or negative. Non‐concordance was defined as undetermined. Results In total, 187 students participated in this study and provided both urine and blood samples. Three students had undetermined rapid u‐HpAb. Excluding these results, the positivity rate of rapid u‐HpAb was 3.3% (6/184), whereas that for u‐Hp ELISA and s‐Hp ELISA was 4.8% (10/187) and 5.9% (11/187), respectively. Using s‐Hp ELISA and u‐Hp ELISA as the standards, the sensitivity, specificity, positive predictive value, and negative predictive value of rapid u‐HpAb were 85.7%, 100%, 100%, and 99.4%, respectively, excluding the undetermined rapid u‐HpAb results. Conclusions Rapid urine‐HpAb test had excellent specificity but relatively low sensitivity.