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Increased expression of CXCR3 axis components and matrix metalloproteinase in pediatric inflammatory bowel disease patients
Author(s) -
Jimbo Keisuke,
Ohtsuka Yoshikazu,
Kojima Yuko,
Hosoi Kenji,
Ohbayashi Naho,
Ikuse Tamaki,
Aoyagi Yo,
Fujii Tohru,
Kudo Takahiro,
Shimizu Toshiaki
Publication year - 2014
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/ped.12362
Subject(s) - lamina propria , medicine , inflammatory bowel disease , cxcl9 , ulcerative colitis , matrix metalloproteinase , pathogenesis , immunohistochemistry , cxcr3 , microarray , immunology , real time polymerase chain reaction , chemokine , reverse transcription polymerase chain reaction , microarray analysis techniques , gene expression , pathology , inflammation , disease , cxcl10 , gene , epithelium , biology , chemokine receptor , biochemistry
Background Although pediatric inflammatory bowel disease ( IBD ) is characterized by extensive intestinal involvement and rapid early progression, the precise cause and specific factors involved in disease aggravation have not been well established. The aim of this study was to investigate the pathogenesis of pediatric IBD . Methods The expression of inflammatory molecules in colon samples taken from active ulcerative colitis ( UC ) and C rohn's disease ( CD ) patients was compared with those of controls. Three children each with UC and CD in both the active and remission phase and their controls were enrolled, and the inflammatory gene expression in the mucosa was examined by microarray. Additionally, six children from each group were further enrolled in a real‐time reverse transcription polymerase chain reaction and an immunohistochemical study to examine the expression of CXCL9 , 10, 11, CXCR3 , matrix metalloproteinase ( MMP )‐1, ‐3, ‐7, and ‐10. Results The microarray analysis revealed enhanced expression of the CXCL9 , 10, and 11 genes in the active phase of CD . The expression of MMP ‐1, ‐3, ‐7, and ‐10 was significantly enhanced in the active phase of UC . These changes were also confirmed by real‐time reverse transcription polymerase chain reaction. Immunohistochemical analysis revealed enhanced expression of CXCL9 , 10, and 11 in both the lamina propria and epithelial cells in these patients. CXCR3 ‐positive cells were also confirmed in the lamina propria. The expression of MMP ‐1, ‐3, ‐7, and ‐10 was also enhanced in the mucosal epithelial cells and the lamina propria in both CD and UC patients. Conclusions These findings suggest that CXCR3 axis components and MMP play an important role in the mucosal damage in pediatric IBD .