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Association of leptin/receptor and TNF ‐α gene variants with adolescent obesity in M alaysia
Author(s) -
Ng Zoe Yi,
Veerapen Muthu Kumar,
Hon Wei Min,
Lim Renee Lay Hong
Publication year - 2014
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/ped.12336
Subject(s) - overweight , medicine , obesity , body mass index , endocrinology , odds ratio , leptin receptor , leptin , genotype , genotyping , genetics , biology , gene
Background Leptin ( LEP ) G ‐2548 A (rs7799039), leptin receptor ( LEPR ) Q 223 R (rs1137101) and tumor necrosis factor ( TNF) ‐α G ‐308 A (rs1800629) gene variants have been reported to be associated with obesity, although results for subjects from different countries have been controversial. The aim of this study was to determine the prevalence of overweight and obesity in M alaysian adolescents and the association of these polymorphisms with overweight and obese or over‐fat adolescents. Methods A total of 613 adolescents (241 M alay, 219 C hinese, 153 I ndian) were enrolled. Anthropometric measurements of body mass index ( BMI) and body fat percentage were used to classify subjects as controls (non‐overweight/obese or normal fat) or as cases (overweight/obese or over‐fat). Genomic DNA was extracted from oral buccal mucosa cells for genotyping using polymerase chain reaction–restriction fragment length polymorphism and data obtained were statistically analyzed. Results A total of 23.3% of subjects were overweight/obese whereas 11.4% were over‐fat; there were significantly more overweight/obese and over‐fat I ndian and M alay adolescents compared to C hinese ( P < 0.001). A allele was the minor one for LEPR Q 223 R and TNF ‐α G ‐308 A in all ethnic groups, whereas G allele was minor for LEP G ‐2548 A in C hinese and M alay adolescents, except for Indian adolescents. Indian male adolescents with AA genotype for LEP G‐2548 A were associated with overweight/obesity ( P = 0.025; odds ratio, 3.64; 95% confidence interval: 1.15–11.54). Despite the lack of association observed for LEPR Q 223 R and TNF ‐α G ‐308 A , I ndian and C hinese subjects with AA risk genotype for LEPR Q 223 R / LEP G ‐2548 A and TNF ‐α G ‐308 A / LEP G ‐2548 A , respectively, had increased mean BMI ( P = 0.049, P = 0.016). Conclusions Genotype distribution and association of these polymorphisms with overweight/obesity vary between ethnic groups and genders. Nevertheless, the LEP G ‐2548 A risk allele may be associated with overweight/obese Indian male adolescents in M alaysia.