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Analysis of inflammatory signals in J apanese children with C rohn's disease
Author(s) -
Fujitake Yoshito,
Ohtsuka Yoshikazu,
Ikuse Tamaki,
Ohtani Kiyotaka,
Aoyagi Yo,
Fujii Tohru,
Kudo Takahiro,
Ishii Masahiro,
Shimizu Toshiaki
Publication year - 2013
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/ped.12159
Subject(s) - medicine , pathogenesis , cytokine , tumor necrosis factor alpha , immunology , acute phase protein , activator (genetics) , gastroenterology , receptor , inflammation
Background Although it is recognized that the T h1 and T h17 cytokines are directly involved in the pathogenesis of C rohn's disease ( CD ), the precise cause of pediatric CD in the J apanese population has not been well established. In the present study, we examined the expression of pro‐inflammatory cytokines and their signaling molecules in the intestinal mucosa of J apanese children with acute‐ and remission‐phase CD . Methods A total of 11 children with acute‐phase CD (mean age 10.32 ± 6.02 years) and 20 children with remission‐phase CD (mean age 11.87 ± 4.29 years) provided samples for a serum cytokine assay. Among these children, seven with acute‐phase CD (mean age 13.63 ± 1.94 years), six with remission‐phase CD (mean age 9.93 ± 4.33 years), and six healthy controls (mean age 9.90 ± 4.88 years) provided samples for a signaling assay. Among this group, the expression of T h1, T h2, T h17, and regulatory T ‐cell signaling molecules were examined by real‐time polymerase chain reaction. Results A significant elevation in the serum level of interleukin‐6 and tumor necrosis factor‐α was confirmed in pediatric patients with acute‐phase CD compared to patients with remission‐phase CD ( P < 0.01 and 0.05, respectively). The mucosal expression of interferon‐γ, signal transducer and activator of transcription 4, and transforming growth factor‐β1 were significantly enhanced in pediatric patients with acute‐phase CD compared to patients with remission‐phase CD or those with normal mucosa. Conclusions These results suggest the possible involvement of T h1 and T h17 signaling in the pathogenesis of CD in Japanese children.