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Kinase gene fusions in defined subsets of melanoma
Author(s) -
Turner Jacqueline,
Couts Kasey,
Sheren Jamie,
Saichaemchan Siriwimon,
Ariyawutyakorn Witthawat,
Avolio Izabela,
Cabral Ethan,
Glogowska Magdelena,
Amato Carol,
Robinson Steven,
Hintzsche Jennifer,
Applegate Allison,
Seelenfreund Eric,
Gonzalez Rita,
Wells Keith,
Bagby Stacey,
Tentler John,
Tan AikChoon,
Wisell Joshua,
VarellaGarcia Marileila,
Robinson William
Publication year - 2017
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/pcmr.12560
Subject(s) - melanoma , fluorescence in situ hybridization , fusion gene , biology , cancer research , gene , immunohistochemistry , ros1 , comparative genomic hybridization , mutation , cancer , genetics , genome , immunology , adenocarcinoma , chromosome
Summary Genomic rearrangements resulting in activating kinase fusions have been increasingly described in a number of cancers including malignant melanoma, but their frequency in specific melanoma subtypes has not been reported. We used break‐apart fluorescence in situ hybridization ( FISH ) to identify genomic rearrangements in tissues from 59 patients with various types of malignant melanoma including acral lentiginous, mucosal, superficial spreading, and nodular. We identified four genomic rearrangements involving the genes BRAF , RET , and ROS 1 . Of these, three were confirmed by Immunohistochemistry (IHC) or sequencing and one was found to be an ARMC 10‐ BRAF fusion that has not been previously reported in melanoma. These fusions occurred in different subtypes of melanoma but all in tumors lacking known driver mutations. Our data suggest gene fusions are more common than previously thought and should be further explored particularly in melanomas lacking known driver mutations.