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Analysis of monosomy‐3 in immunomagnetically isolated circulating melanoma cells in uveal melanoma patients
Author(s) -
Tura Aysegül,
Merz Hartmut,
Reinsberg Mihaela,
Lüke Matthias,
Jager Martine J.,
Grisanti Salvatore,
Lüke Julia
Publication year - 2016
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/pcmr.12507
Subject(s) - monosomy , melanoma , metastasis , cancer research , medicine , oncology , pathology , biology , karyotype , chromosome , cancer , genetics , gene
Summary Monosomy‐3 in primary uveal melanoma ( UM ) is associated with a high risk of metastasis and mortality. Although circulating melanoma cells ( CMC ) can be found in most UM patients, only approximately 50% of the patients develop metastases. We utilized a novel immuno‐ FISH assay to detect chromosome‐3 in intact CMC isolated by dual immunomagnetic enrichment. Circulating melanoma cells were detected in 91% of the patients (n = 44) with primary non‐metastatic UM , of which 58% were positive for monosomy‐3. The monosomy‐3 status of CMC corresponded to the monosomy‐3 status of the primary tumor in 10 of the 11 patients where this could be tested. Monosomy‐3 in the CMC was associated with an advanced tumor stage (P = 0.046) and was detected in all four patients who developed metastasis within the follow‐up period of 4 yr. This non‐invasive technique may enable the identification of UM patients at risk for metastasis particularly when a primary tumor specimen is unavailable.