Premium
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Author(s) -
Lazar Ikrame,
Clement Emily,
DucouxPetit Manuelle,
Denat Laurence,
Soldan Vanessa,
Dauvillier Stéphanie,
Balor Stéphanie,
BurletSchiltz Odile,
Larue Lionel,
Muller Catherine,
Nieto Laurence
Publication year - 2015
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/pcmr.12380
Subject(s) - microvesicles , melanoma , biology , proteome , microbiology and biotechnology , cell culture , angiogenesis , cancer research , cell , exosome , tumor microenvironment , immune system , immunology , microrna , genetics , gene
Summary Exosomes are important mediators in cell‐to‐cell communication and, recently, their role in melanoma progression has been brought to light. Here, we characterized exosomes secreted by seven melanoma cell lines with varying degrees of aggressivity. Extensive proteomic analysis of their exosomes confirmed the presence of characteristic exosomal markers as well as melanoma‐specific antigens and oncogenic proteins. Importantly, the protein composition differed among exosomes from different lines. Exosomes from aggressive cells contained specific proteins involved in cell motility, angiogenesis, and immune response, while these proteins were less abundant or absent in exosomes from less aggressive cells. Interestingly, when exposed to exosomes from metastatic lines, less aggressive cells increased their migratory capacities, likely due to transfer of pro‐migratory exosomal proteins to recipient cells. Hence, this study shows that the specific protein composition of melanoma exosomes depends on the cells’ aggressivity and suggests that exosomes influence the behavior of other tumor cells and their microenvironment.