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DNA methylation profiles in primary cutaneous melanomas are associated with clinically significant pathologic features
Author(s) -
Thomas Nancy E.,
Slater Nathaniel A.,
Edmiston Sharon N.,
Zhou Xin,
Kuan PeiFen,
Groben Pamela A.,
Carson Craig C.,
Hao Honglin,
Parrish Eloise,
Moschos Stergios J.,
Berwick Marianne,
Ollila David W.,
Conway Kathleen
Publication year - 2014
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/pcmr.12289
Subject(s) - methylation , dna methylation , melanoma , breslow thickness , cpg site , biology , cancer research , pathology , cancer , genetics , medicine , gene , gene expression , breast cancer , sentinel lymph node
Summary DNA methylation studies have elucidated a methylation signature distinguishing primary melanomas from benign nevi and provided new insights about genes that may be important in melanoma development. However, it is unclear whether methylation differences among primary melanomas are related to tumor pathologic features with known clinical significance. We utilized the Illumina GoldenGate Cancer Panel array to investigate the methylation profiles of 47 primary cutaneous melanomas. Arraywide methylation patterns revealed a positive association of methylation with Breslow thickness and mutated BRAF , a negative association with mitotic rate, and a weak association with ulceration. Hierarchical clustering on CpG sites exhibiting the most variable methylation (n = 235) divided the melanoma samples into three clusters, including a highly methylated cluster that was positively associated with Breslow thickness and an intermediately methylated cluster associated with Breslow thickness and mitotic rate. Our findings provide support for the existence of methylation‐defined subsets in melanomas with increased methylation associated with Breslow thickness.