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miR‐20b regulates expression of proteinase‐activated receptor‐1 (PAR‐1) thrombin receptor in melanoma cells
Author(s) -
Saleiban Amina,
Faxälv Lars,
Claesson Kjersti,
Jönsson JanIngvar,
Osman Abdimajid
Publication year - 2014
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/pcmr.12217
Subject(s) - melanoma , transfection , cancer research , microbiology and biotechnology , microrna , luciferase , biology , downregulation and upregulation , receptor , cell culture , gene , biochemistry , genetics
Summary The proteinase‐activated receptor 1 ( PAR ‐1) plays a central role in melanoma progression and its expression level is believed to correlate with the degree of cancer invasiveness. Here, we show that PAR ‐1 is post‐transcriptionally regulated by miR‐20b micro RNA in human melanoma cells. PAR ‐1 was found to be expressed in metastatic melanoma cells but was barely detectable in primary melanoma. By transducing primary melanoma cells with a lentivirus containing a 3′‐ UTR construct of PAR ‐1 mRNA , we could show that endogenous melanoma micro RNA s interacted with PAR ‐1 3′‐ UTR and silenced a fused luciferase reporter. Transfection of an inhibitor against miR‐20b into primary melanoma cells reversed this process. Finally, transfection of miR‐20b mimic into metastatic melanoma cells caused downregulation of the luciferase reporter. We conclude that miR‐20b regulates expression of melanoma PAR ‐1 receptor, which may explain the differential expression of PAR ‐1 observed in human melanoma.