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Pulsed ultrasound promotes melanoblast migration through upregulation of macrophage colony‐stimulating factor/focal adhesion kinase autocrine signaling and paracrine mechanisms
Author(s) -
Liao YiHua,
Huang YuTing,
Deng JhuYun,
Chen WenShiang,
Jee ShiouHwa
Publication year - 2013
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/pcmr.12125
Subject(s) - paracrine signalling , autocrine signalling , focal adhesion , microbiology and biotechnology , melanocyte , cell migration , cancer research , cell growth , biology , ptk2 , signal transduction , cell culture , protein kinase c , mitogen activated protein kinase kinase , receptor , melanoma , biochemistry , genetics
Summary Repigmentation of vitiliginous lesions relies on the proliferation and migration of melanoblasts from hair follicles to the epidermis. Pulsed ultrasound has been demonstrated to have stimulatory effects on cell proliferation and migration and has been applied clinically to enhance tissue repair. To clarify the biologic effects and signaling mechanisms of pulsed ultrasound on melanoblast proliferation and migration, two melanoblast cell lines, the undifferentiated NCC melb4 cells and the differentiated NCC melan5 cells, were examined. We demonstrated that pulsed ultrasound increased cell migration in a dose‐dependent manner without altering cell proliferation. Pulsed ultrasound enhanced autocrine secretion of macrophage colony‐stimulating factor ( M ‐ CSF ), which subsequently activated the focal adhesion kinase ( FAK ) pathway to promote melanoblast migration. Furthermore, conditioned medium from mouse embryonic fibroblasts NIH 3T3 and primary human keratinocytes treated with pulsed ultrasound could stimulate melanoblast migration through a paracrine effect. Our results provide a novel mechanism to promote migration of melanoblasts by pulsed ultrasound stimulation.