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The mouse ruby‐eye 2 d ( ru2 d / H ps5 ru2‐d ) allele inhibits eumelanin but not pheomelanin synthesis
Author(s) -
Hirobe Tomohisa,
Ito Shosuke,
Wakamatsu Kazumasa
Publication year - 2013
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/pcmr.12118
Subject(s) - congenic , microbiology and biotechnology , allele , melanin , chemistry , mutation , biology , genetics , gene
Summary The novel mutation named ru2 d / H ps5 ru2‐d , characterized by light‐colored coats and ruby‐eyes, prohibits differentiation of melanocytes by inhibiting tyrosinase ( T yr) activity, expression of T yr, T yr‐related protein 1 ( T yrp1), T yrp2, and Kit. However, it is not known whether the ru2 d allele affects pheomelanin synthesis in recessive yellow ( e/ M c1r e ) or in pheomelanic stage in agouti ( A ) mice. In this study, effects of the ru2 d allele on pheomelanin synthesis were investigated by chemical analysis of melanin present in dorsal hairs of 5‐week‐old mice from F 2 generation between C 57 BL /10 JH ir ( B 10)‐co‐isogenic ruby‐eye 2 d and B 10‐congenic recessive yellow or agouti. Eumelanin content was decreased in ruby‐eye 2 d and ruby‐eye 2 d agouti mice, whereas pheomelanin content in ruby‐eye 2 d recessive yellow and ruby‐eye 2 d agouti mice did not differ from the corresponding R u2 d /‐ mice, suggesting that the ru2 d allele inhibits eumelanin but not pheomelanin synthesis.