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Resistance to cisplatin‐induced cell death conferred by the activity of organic anion transporting polypeptides ( OATP ) in human melanoma cells
Author(s) -
Silvy Françoise,
Lissitzky JeanClaude,
Bruneau Nadine,
Zucchini Nathalie,
Landrier JeanFrançois,
Lombardo Dominique,
Verrando Patrick
Publication year - 2013
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/pcmr.12108
Subject(s) - melanoma , organic anion transporting polypeptide , cisplatin , apoptosis , chemistry , efflux , organic anion transporter 1 , transporter , cancer research , microbiology and biotechnology , biology , biochemistry , gene , genetics , chemotherapy
Summary Expression of organic anion transporting polypeptides (OATP) transporters can be modified with potential incidence in cancers, yet they have not been considered in melanoma. Here, we demonstrate transcriptional and protein expression of OATP members in human melanoma cell lines with sodium‐independent organic anion uptake activity. Importantly, uptake of different organic anions over 24 h led to a common resistance signal to apoptotic cell death, induced further by cisplatin in 24 h. The mechanism is not dependent on the transport of cisplatin by the OATP , as it is not an OATP substrate. The resistance signal was modulated by PKC , disclosing it as signal mediator. This study suggests that OATP , which can be constantly activated by endobiotics, may contribute to melanoma chemotherapeutic resistance, thereby justifying the development of OATP targeting strategies.