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GmST1 , which encodes a sulfotransferase, confers resistance to soybean mosaic virus strains G2 and G3
Author(s) -
Zhao Xue,
Jing Yan,
Luo Zhenghui,
Gao Sainan,
Teng Weili,
Zhan Yuhang,
Qiu Lijuan,
Zheng Hongkun,
Li Wenbin,
Han Yingpeng
Publication year - 2021
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.14066
Subject(s) - chemistry , biology , virology
Soybean mosaic virus (SMV) is one of the most widespread and devastating viral diseases worldwide. The genetic architecture of qualitative resistance to SMV in soybean remains unclear. Here, the Rsvg2 locus was identified as underlying soybean resistance to SMV by genome‐wide association and linkage analyses. Fine mapping results showed that soybean resistance to SMV strains G2 and G3 was controlled by a single dominant gene, GmST1 , on chromosome 13, encoding a sulfotransferase (SOT). A key variation at position 506 in the coding region of GmST1 associated with the structure of the encoded SOT and changed SOT activity levels between RSVG2‐S and RSVG2‐R alleles. In RSVG2‐S allele carrier “Hefeng25”, the overexpression of GmST1 carrying the RSVG2‐R allele from the SMV‐resistant line “Dongnong93‐046” conferred resistance to SMV strains G2 and G3. Compared to Hefeng25, the accumulation of SMV was decreased in transgenic plants carrying the RSVG2‐R allele. SMV infection differentiated both the accumulation of jasmonates and expression patterns of genes involved in jasmonic acid (JA) signalling, biosynthesis and catabolism in RSVG2‐R and RSVG2‐S allele carriers. This characterization of GmST1 suggests a new scenario explaining soybean resistance to SMV.