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Root isoprene formation alters lateral root development
Author(s) -
Miloradovic van Doorn Maja,
MerlPham Juliane,
Ghirardo Andrea,
Fink Siegfried,
Polle Andrea,
Schnitzler JörgPeter,
Rosenkranz Maaria
Publication year - 2020
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13814
Subject(s) - isoprene , chemistry , reactive oxygen species , auxin , peroxidase , vascular tissue , botany , biochemistry , biology , enzyme , gene , organic chemistry , copolymer , polymer
Isoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short‐term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analysing Populus x canescens isoprene synthase ( PcISPS ) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non‐emitting poplar roots revealed an accumulation of O 2 − in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signalling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S‐lipoxygenase, in isoprene non‐emitting poplar roots. Together our results indicate for isoprene a ROS‐related function, eventually co‐regulating the plant‐internal signalling network and development processes in root tissue.