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A multiscale approach reveals regulatory players of water stress responses in seeds during germination
Author(s) -
Saux Marine,
Ponnaiah Maharajah,
Langlade Nicolas,
Zanchetta Catherine,
Balliau Thierry,
ElMaaroufBouteau Hayat,
Bailly Christophe
Publication year - 2020
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13731
Subject(s) - germination , transcriptome , helianthus annuus , biology , heat shock protein , sunflower , proteome , reactive oxygen species , sunflower seed , water stress , hybrid , osmotic shock , botany , horticulture , agronomy , microbiology and biotechnology , gene expression , biochemistry , gene
Seed germination is regulated by environmental factors, particularly water availability. Water deficits at the time of sowing impair the establishment of crop plants. Transcriptome and proteome profiling was used to document the responses of sunflower ( Helianthus annuus ) seeds to moderate water stress during germination in two hybrids that are nominally classed as drought sensitive and drought tolerant. Differences in the water stress‐dependent accumulation reactive oxygen species and antioxidant enzymes activities were observed between the hybrids. A pathway‐based analysis of the hybrid transcriptomes demonstrated that the water stress‐dependent responses of seed metabolism were similar to those of the plant, with a decreased abundance of transcripts encoding proteins associated with metabolism and cell expansion. Moreover, germination under water stress conditions was associated with increased levels of transcripts encoding heat shock proteins. Exposure of germinating seeds to water stress specifically affected the abundance of a small number of proteins, including heat shock proteins. Taken together, these data not only identify factors that are likely to play a key role in drought tolerance during seed germination, but they also demonstrate the importance of the female parent in the transmission of water stress tolerance.