Premium
GsSnRK1 interplays with transcription factor GsERF7 from wild soybean to regulate soybean stress resistance
Author(s) -
Feng Xu,
Feng Peng,
Yu Huilin,
Yu Xingyu,
Sun Qi,
Liu Siyu,
Minh Thuy Nguyen,
Chen Jun,
Wang Di,
Zhang Qing,
Cao Lei,
Zhou Changmei,
Li Qiang,
Xiao Jialei,
Zhong Shihua,
Wang Aoxue,
Wang Lijuan,
Pan Hongyu,
Ding Xiaodong
Publication year - 2020
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13726
Subject(s) - transactivation , abiotic stress , transcription factor , microbiology and biotechnology , biology , gene , kinase , transgene , abiotic component , phosphorylation , botany , biochemistry , paleontology
Although the function and regulation of SnRK1 have been studied in various plants, its molecular mechanisms in response to abiotic stresses are still elusive. In this work, we identified an AP2/ERF domain‐containing protein (designated GsERF7) interacting with GsSnRK1 from a wild soybean cDNA library. GsERF7 gene expressed dominantly in wild soybean roots and was responsive to ethylene, salt, and alkaline. GsERF7 bound GCC cis ‐acting element and could be phosphorylated on S36 by GsSnRK1. GsERF7 phosphorylation facilitated its translocation from cytoplasm to nucleus and enhanced its transactivation activity. When coexpressed in the hairy roots of soybean seedlings, GsSnRK1(wt) and GsERF7(wt) promoted plants to generate higher tolerance to salt and alkaline stresses than their mutated species, suggesting that GsSnRK1 may function as a biochemical and genetic upstream kinase of GsERF7 to regulate plant adaptation to environmental stresses. Furthermore, the altered expression patterns of representative abiotic stress‐responsive and hormone‐synthetic genes were determined in transgenic soybean hairy roots after stress treatments. These results will aid our understanding of molecular mechanism of how SnRK1 kinase plays a cardinal role in regulating plant stress resistances through activating the biological functions of downstream factors.