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Mid‐infrared spectroscopy is a fast screening method for selecting Arabidopsis genotypes with altered leaf cuticular wax
Author(s) -
Liu Na,
Zhao Lifang,
Tang Lily,
Stobbs Jarvis,
Parkin Isobel,
Kunst Ljerka,
Karunakaran Chithra
Publication year - 2020
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13691
Subject(s) - wax , attenuated total reflection , fourier transform infrared spectroscopy , plant cuticle , cuticle (hair) , infrared spectroscopy , arabidopsis , chemistry , spectroscopy , arabidopsis thaliana , chemical composition , botany , mutant , horticulture , analytical chemistry (journal) , biology , chromatography , biochemistry , organic chemistry , chemical engineering , gene , genetics , physics , quantum mechanics , engineering
Arabidopsis eceriferum ( cer ) mutants with unique alterations in their rosette leaf cuticular wax accumulation and composition established by gas chromatography have been investigated using attenuated total reflection (ATR)‐Fourier transform infrared (FTIR) spectroscopy in combination with univariate and multivariate analysis. Objectives of this study were to evaluate the utility of ATR‐FTIR for detection of chemical diversity in leaf cuticles, obtain spectral profiles of cer mutants in comparison with the wild type, and identify changes in leaf cuticles caused by drought stress. FTIR spectra revealed both genotype‐ and treatment‐dependent differences in the chemical make‐up of Arabidopsis leaf cuticles. Drought stress caused specific changes in the integrated area of the CH 3 peak, asymmetrical and symmetrical CH 2 peaks, ester carbonyl peak and the peak area ratio of ester CO to CH 2 asymmetrical vibration. CH 3 peak positively correlated with the total wax accumulation. Thus, ATR‐FTIR spectroscopy is a valuable tool that can advance our understanding of the role of cuticle chemistry in plant response to drought and allow selection of superior drought‐tolerant varieties from large genetic resources.