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Comparative spatial lipidomics analysis reveals cellular lipid remodelling in different developmental zones of barley roots in response to salinity
Author(s) -
Sarabia Lenin D.,
Boughton Berin A.,
Rupasinghe Thusitha,
Callahan Damien L.,
Hill Camilla B.,
Roessner Ute
Publication year - 2020
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13653
Subject(s) - lipidomics , chemistry , halophyte , phosphatidylcholine , mass spectrometry , hordeum vulgare , mass spectrometry imaging , salinity , tandem mass spectrometry , biochemistry , chromatography , botany , biology , phospholipid , poaceae , ecology , membrane
Salinity‐induced metabolic, ionic, and transcript modifications in plants have routinely been studied using whole plant tissues, which do not provide information on spatial tissue responses. The aim of this study was to assess the changes in the lipid profiles in a spatial manner and to quantify the changes in the elemental composition in roots of seedlings of four barley cultivars before and after a short‐term salt stress. We used a combination of liquid chromatography–tandem mass spectrometry, inductively coupled plasma mass spectrometry, matrix‐assisted laser desorption/ionization mass spectrometry imaging, and reverse transcription – quantitative real time polymerase chain reaction platforms to examine the molecular signatures of lipids, ions, and transcripts in three anatomically different seminal root tissues before and after salt stress. We found significant changes to the levels of major lipid classes including a decrease in the levels of lysoglycerophospholipids, ceramides, and hexosylceramides and an increase in the levels of glycerophospholipids, hydroxylated ceramides, and hexosylceramides. Our results revealed that modifications to lipid and transcript profiles in plant roots in response to a short‐term salt stress may involve recycling of major lipid species, such as phosphatidylcholine, via resynthesis from glycerophosphocholine.