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The “one‐point method” for estimating maximum carboxylation capacity of photosynthesis: A cautionary tale
Author(s) -
Burnett Angela C.,
Davidson Kenneth J.,
Serbin Shawn P.,
Rogers Alistair
Publication year - 2019
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13574
Subject(s) - carboxylation , photosynthesis , rubisco , acclimatization , irradiance , compensation point , respiration , mathematics , botany , chemistry , statistics , physics , biology , biochemistry , optics , transpiration , catalysis
The maximum carboxylation capacity of Rubisco, V c,max , is an important photosynthetic parameter that is key to accurate estimation of carbon assimilation. The gold‐standard technique for determining V c,max is to derive V c,max from the initial slope of an A – C i curve (the response of photosynthesis, A , to intercellular CO 2 concentration, C i ). Accurate estimates of V c,max derived from an alternative and rapid “one‐point” measurement of photosynthesis could greatly accelerate data collection and model parameterization. We evaluated the practical application of the one‐point method in six species measured under standard conditions (saturating irradiance and 400 μmol CO 2 mol −1 ) and under conditions that would increase the likelihood for successful estimation of V c,max : (a) ensuring Rubisco‐limited A by measuring at 300 μmol CO 2 mol −1 and (b) allowing time for acclimation to saturating irradiance prior to measurement. The one‐point method significantly underestimated V c,max in four of the six species, providing estimates 21%–32% below fitted values. We identified ribulose‐1,5‐bisphosphate‐limited A , light acclimation, and the use of an assumed respiration rate as factors that limited the effective use of the one‐point method to accurately estimate V c,max . We conclude that the one‐point method requires a species‐specific understanding of its application, is often unsuccessful, and must be used with caution.