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EgRBP 42 from oil palm enhances adaptation to stress in Arabidopsis through regulation of nucleocytoplasmic transport of stress‐responsive mRNAs
Author(s) -
Yeap WanChin,
Namasivayam Parameswari,
Ooi Tony Eng Keong,
Appleton David Ross,
Kulaveerasingam Harikrishna,
Ho ChaiLing
Publication year - 2019
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13503
Subject(s) - microbiology and biotechnology , heterogeneous nuclear ribonucleoprotein , arabidopsis , abiotic stress , biology , ribonucleoprotein , rna binding protein , rna , gene expression , gene , genetics , mutant
Abiotic stress reduces plant growth and crop productivity. However, the mechanism underlying posttranscriptional regulations of stress response remains elusive. Herein, we report the posttranscriptional mechanism of nucleocytoplasmic RNA transport of stress‐responsive transcripts mediated by EgRBP42, a heterogeneous nuclear ribonucleoprotein‐like RNA‐binding protein from oil palm, which could be necessary for rapid protein translation to confer abiotic stress tolerance in plants. Transgenic Arabidopsis overexpressing EgRBP42 showed early flowering through alteration of gene expression of flowering regulators and exhibited tolerance towards heat, cold, drought, flood, and salinity stresses with enhanced poststress recovery response by increasing the expression of its target stress‐responsive genes. EgRBP42 harbours nucleocytoplasmic shuttling activity mediated by the nuclear localization signal and the M9‐like domain of EgRBP42 and interacts directly with regulators in the nucleus, membrane, and the cytoplasm. EgRBP42 regulates the nucleocytoplasmic RNA transport of target stress‐responsive transcripts through direct binding to their AG‐rich motifs. Additionally, EgRBP42 transcript and protein induction by environmental stimuli are regulated at the transcriptional and posttranscriptional levels. Taken together, the posttranscriptional regulation of RNA transport mediated by EgRBP42 may change the stress‐responsive protein profiles under abiotic stress conditions leading to a better adaptation of plants to environmental changes.