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Hydrogen peroxide facilitates Arabidopsis seedling establishment by interacting with light signalling pathway in the dark
Author(s) -
Cheng Han,
Liang Qun,
Chen Xiang,
Zhang Yuanyuan,
Qiao Fei,
Guo Dianjing
Publication year - 2019
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13482
Subject(s) - photomorphogenesis , arabidopsis , seedling , biology , phytochrome , botany , microbiology and biotechnology , biochemistry , gene , mutant , red light
Light is essential for the plant establishment. Arabidopsis seedlings germinated in the dark cannot grow leaf and only have closed cotyledons. However, exogenous application of H 2 O 2 can induce leaves (establishment) in the dark. Comparative transcriptomic analysis revealed that light‐responsive genes were activated by H 2 O 2 treatment. These genes are functionally correlated with photosynthesis, photorespiration, and components of photosystem, such as antenna proteins and light‐harvesting chlorophyll proteins. We further found that application of H 2 O 2 facilitates cell cycle by accelerating G 2 –M checkpoint transition in shoot apical meristem. Phytochrome‐mediated light signalling pathway was also involved in the H 2 O 2 ‐facilitated establishment process. The constitutive photomorphogenesis 1 and phytochrome interacting factor 3 proteins were shown to be down‐regulated by H 2 O 2 treatment and accordingly removed their inhibitory effects on photomorphogenesis in the dark. The crosstalk between oxidation and light signal pathways explains the mechanism that H 2 O 2 regulates plant dark establishment. The endogenous photorespiratory H 2 O 2 production was mimicked by overexpression of glycolate oxidase genes and supplement of substrate glycolate. As expected, seedling establishment was also induced by the endogenously produced H 2 O 2 under dark condition. These findings also suggest that photorespiratory H 2 O 2 production is at least partially involved in postgermination establishment.