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Understanding genetic control of root system architecture in soybean: Insights into the genetic basis of lateral root number
Author(s) -
Prince Silvas J.,
Valliyodan Babu,
Ye Heng,
Yang Ming,
Tai Shuaishuai,
Hu Wushu,
Murphy Mackensie,
Durnell Lorellin A.,
Song Li,
Joshi Trupti,
Liu Yang,
Van de Velde Jan,
Vandepoele Klaas,
Grover Shan J.,
Nguyen Henry T.
Publication year - 2019
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13333
Subject(s) - biology , germplasm , single nucleotide polymorphism , genetics , gene , genetic diversity , genetic variation , root system , lateral root , genome wide association study , association mapping , genotype , botany , arabidopsis , mutant , population , demography , sociology
Abstract Developing crops with better root systems is a promising strategy to ensure productivity in both optimum and stress environments. Root system architectural traits in 397 soybean accessions were characterized and a high‐density single nucleotide polymorphisms (SNPs)‐based genome‐wide association study was performed to identify the underlying genes associated with root structure. SNPs associated with root architectural traits specific to landraces and elite germplasm pools were detected. Four loci were detected in landraces for lateral root number (LRN) and distribution of root thickness in diameter Class I with a major locus on chromosome 16. This major loci was detected in the coding region of unknown protein, and subsequent analyses demonstrated that root traits are affected with mutated haplotypes of the gene. In elite germplasm pool, 3 significant SNPs in alanine‐glyoxalate aminotransferase, Leucine‐Rich Repeat receptor/No apical meristem, and unknown functional genes were found to govern multiple traits including root surface area and volume. However, no major loci were detected for LRN in elite germplasm. Nucleotide diversity analysis found evidence of selective sweeps around the landraces LRN gene. Soybean accessions with minor and mutated allelic variants of LRN gene were found to perform better in both water‐limited and optimal field conditions.

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