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OsPRR37 confers an expanded regulation of the diurnal rhythms of the transcriptome and photoperiodic flowering pathways in rice
Author(s) -
Liu Chuan,
Qu Xuefeng,
Zhou Yanhao,
Song Gaoyuan,
Abiri Naghmeh,
Xiao Yuhui,
Liang Fan,
Jiang Daiming,
Hu Zhongli,
Yang Daichang
Publication year - 2018
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13135
Subject(s) - circadian rhythm , biology , transcriptome , circadian clock , clock , gene , microbiology and biotechnology , genetics , gene expression , endocrinology
The circadian clock enables organisms to rapidly adapt to the ever‐changing environmental conditions that are caused by daily light/dark cycles. Circadian clock genes universally affect key agricultural traits, particularly flowering time. Here, we show that OsPRR37 , a circadian clock gene, delays rice flowering time in an expression level‐dependent manner. Using high‐throughput mRNA sequencing on an OsPRR37 overexpressing transgenic line ( OsPRR37 ‐OE5) and the recipient parent Guangluai4 that contains the loss‐of‐function Osprr37 , we identify 14,992 genes that display diurnal rhythms, which account for 52.9% of the transcriptome. Overexpressing OsPRR37 weakens the transcriptomic rhythms and alters the phases of rhythmic genes. In total, 3,210 differentially expressed genes (DEGs) are identified, among which 1,863 rhythmic DEGs show a correlation between the change of absolute amplitudes and the mean expression levels. We further reveal that OsPRR37 functions as a transcriptional repressor to repress the expression levels and amplitudes of day‐phased clock genes. More importantly, OsPRR37 confers expanded regulation on the evening‐phased rhythmic DEGs by repressing the morning‐phased rhythmic DEGs. Further study shows that OsPRR37 expands its regulation on flowering pathways by repressing Ehd1 . Thus, our results demonstrate an expanded regulation mechanism of the circadian clock on the diurnal rhythms of the transcriptome.