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Implementation of CsLIS/NES in linalool biosynthesis involves transcript splicing regulation in Camellia sinensis
Author(s) -
Liu GuoFeng,
Liu JingJing,
He ZhiRong,
Wang FuMin,
Yang Hua,
Yan YiFeng,
Gao MingJun,
Gruber Margaret Y.,
Wan XiaoChun,
Wei Shu
Publication year - 2018
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13080
Subject(s) - linalool , methyl jasmonate , nerolidol , camellia sinensis , biology , phenylpropanoid , biochemistry , jasmonate , rna splicing , biosynthesis , gene expression , alternative splicing , gene , messenger rna , botany , arabidopsis , mutant , rna , essential oil
Volatile terpenoids produced in tea plants ( Camellia sinensis ) are airborne signals interacting against other ecosystem members, but also pleasant odorants of tea products. Transcription regulation (including transcript processing) is pivotal for plant volatile terpenoid production. In this study, a terpene synthase gene CsLIS/NES was recovered from tea plants ( C . sinensis cv. “Long‐Men Xiang”). CsLIS/NES transcription regulation resulted in 2 splicing forms: CsLIS/NES‐1 and CsLIS/NES‐2 lacking a 305 bp ‐fragment at N‐terminus, both producing ( E )‐nerolidol and linalool in vitro . Transgenic tobacco studies and a gene‐specific antisense oligo‐deoxynucleotide suppression applied in tea leaves indicated that CsLIS/NES‐1 , localized in chloroplasts, acted as linalool synthase, whereas CsLIS/NES‐2 localized in cytosol, functioned as a potential nerolidol synthase, but not linalool synthase. Expression patterns of the 2 transcript isoforms in tea were distinctly different and responded differentially to the application of stress signal molecule methyl jasmonate. Leaf expression of CsLIS/NES ‐ 1 , but not CsLIS/NES ‐ 2 , was significantly induced by methyl jasmonate. Our data indicated that distinct transcript splicing regulation patterns, together with subcellular compartmentation of CsLIS/NE‐1 and CsLIS/NE‐2 implemented the linalool biosynthesis regulation in tea plants in responding to endogenous and exogenous regulatory factors.