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Cytosolic glyceraldehyde‐3‐phosphate dehydrogenases play crucial roles in controlling cold‐induced sweetening and apical dominance of potato ( Solanum tuberosum L.) tubers
Author(s) -
Liu Tengfei,
Fang Hui,
Liu Jun,
Reid Stephen,
Hou Juan,
Zhou Tingting,
Tian Zhendong,
Song Botao,
Xie Conghua
Publication year - 2017
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13073
Subject(s) - meristem , apical dominance , glyceraldehyde 3 phosphate dehydrogenase , biology , dehydrogenase , solanum tuberosum , gene , microbiology and biotechnology , cytosol , biochemistry , sucrose synthase , glycolysis , sucrose , enzyme , botany , invertase , shoot
Abstract Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) is an important enzyme that functions in producing energy and supplying intermediates for cellular metabolism. Recent researches indicate that GAPDHs have multiple functions beside glycolysis. However, little information is available for functions of GAPDHs in potato. Here, we identified 4 putative cytosolic GAPDH genes in potato genome and demonstrated that the StGAPC1, StGAPC2, and StGAPC3, which are constitutively expressed in potato tissues and cold inducible in tubers, encode active cytosolic GAPDHs. Cosuppression of these 3 GAPC genes resulted in low tuber GAPDH activity, consequently the accumulation of reducing sugars in cold stored tubers by altering the tuber metabolite pool sizes favoring the sucrose pathway. Furthermore, GAPC s‐silenced tubers exhibited a loss of apical dominance dependent on cell death of tuber apical bud meristem (TAB‐meristem). It was also confirmed that StGAPC1, StGAPC2, and StGAPC3 interacted with the autophagy‐related protein 3 (ATG3), implying that the occurrence of cell death in TAB‐meristem could be induced by ATG3 associated events. Collectively, the present research evidences first that the GAPC genes play crucial roles in diverse physiological and developmental processes in potato tubers.