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Phospholipase Dδ negatively regulates plant thermotolerance by destabilizing cortical microtubules in Arabidopsis
Author(s) -
Zhang Qun,
Song Ping,
Qu Yana,
Wang Peipei,
Jia Qianru,
Guo Liang,
Zhang Chuanpeng,
Mao Tonglin,
Yuan Ming,
Wang Xuemin,
Zhang Wenhua
Publication year - 2017
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.13023
Subject(s) - microtubule , arabidopsis , phospholipase d , microbiology and biotechnology , biology , proteostasis , chemistry , mutant , biochemistry , signal transduction , gene
The pattern of cortical microtubule arrays plays an important role in plant growth and adaptation in response to hormonal and environmental changes. Cortical microtubules are connected with the plasma membrane (PM); however, how the membrane affects cortical microtubule organization is not well understood. Here, we showed that phospholipase Dδ (PLDδ) was associated with the PM and co‐localized with microtubules in cells. In vitro analysis revealed that PLDδ bound to microtubules, resulting in microtubule disorganization. Site‐specific mutations that decreased PLDδ enzymatic activity impaired its effects on destabilizing microtubule organization. Heat shock transiently activated PLDδ, without any change of its PM localization, triggering microtubule dissociation from PM and depolymerization and seedling death in Arabidopsis , but these effects were alleviated in pldδ knockout mutants. Complementation of pldδ with wild‐type PLDδ , but not mutated PLDδ , restored the phenotypes of microtubules and seedling survival to those of wild‐type Arabidopsis . Thus, we conclude that the PM‐associated PLDδ negatively regulates plant thermotolerance via destabilizing cortical microtubules, in an activity‐dependent manner, rather than its subcellular translocation.