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A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short‐arm of chromosome 3
Author(s) -
Watanabe Kohtaro,
Takahashi Hirokazu,
Sato Saori,
Nishiuchi Shunsaku,
Omori Fumie,
Malik Al Imran,
Colmer Timothy David,
Mano Yoshiro,
Nakazono Mikio
Publication year - 2017
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12849
Subject(s) - suberin , apoplast , aerenchyma , biology , epidermis (zoology) , botany , lignin , endodermis , biophysics , anatomy , cell wall
A radial oxygen loss (ROL) barrier in roots of waterlogging‐tolerant plants promotes oxygen movement via aerenchyma to the root tip, and impedes soil phytotoxin entry. The molecular mechanism and genetic regulation of ROL barrier formation are largely unknown. Zea nicaraguensis , a waterlogging‐tolerant wild relative of maize ( Zea mays ssp. mays ), forms a tight ROL barrier in its roots when waterlogged. We used Z . nicaraguensis chromosome segment introgression lines (ILs) in maize (inbred line Mi29) to elucidate the chromosomal region involved in regulating root ROL barrier formation. A segment of the short‐arm of chromosome 3 of Z . nicaraguensis conferred ROL barrier formation in the genetic background of maize. This chromosome segment also decreased apoplastic solute permeability across the hypodermis/exodermis. However, the IL and maize were similar for suberin staining in the hypodermis/exodermis at 40 mm and further behind the root tip. Z . nicaraguensis contained suberin in the hypodermis/exodermis at 20 mm and lignin at the epidermis. The IL with ROL barrier, however, did not contain lignin in the epidermis. Discovery of the Z . nicaraguensis chromosomal region responsible for root ROL barrier formation has improved knowledge of this trait and is an important step towards improvement of waterlogging tolerance in maize.