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Biochemical basis of sulphenomics: how protein sulphenic acids may be stabilized by the protein microenvironment
Author(s) -
Trost P.,
Fermani S.,
Calvaresi M.,
Zaffagnini M.
Publication year - 2017
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12791
Subject(s) - cysteine , chemistry , reactivity (psychology) , hydrogen peroxide , amino acid , deprotonation , thiol , sulfenic acid , biochemistry , stereochemistry , combinatorial chemistry , enzyme , organic chemistry , medicine , ion , alternative medicine , pathology
Among protein residues, cysteines are one of the prominent candidates to ROS‐mediated and RNS‐mediated post‐translational modifications, and hydrogen peroxide (H 2 O 2 ) is the main ROS candidate for inducing cysteine oxidation. The reaction with H 2 O 2 is not common to all cysteine residues, being their reactivity an utmost prerequisite for the sensitivity towards H 2 O 2 . Indeed, only deprotonated Cys (i.e. thiolate form, S − ) can react with H 2 O 2 leading to sulphenic acid formation (SOH), which is considered as a major/central player of ROS sensing pathways. However, cysteine sulphenic acids are generally unstable because they can be further oxidized to irreversible forms (sulphinic and sulphonic acids, SO 2 H and SO 3 H, respectively), or alternatively, they can proceed towards further modifications including disulphide bond formation (SS), S‐glutathionylation (SSG) and sulphenamide formation (SN). To understand why and how cysteine residues undergo primary oxidation to sulphenic acid, and to explore the stability of cysteine sulphenic acids, a combination of biochemical, structural and computational studies are required. Here, we will discuss the current knowledge of the structural determinants for cysteine reactivity and sulphenic acid stability within protein microenvironments.