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Nitric oxide‐fixation by non‐symbiotic haemoglobin proteins in Arabidopsis thaliana under N‐limited conditions
Author(s) -
Kuruthukulangarakoola Gitto Thomas,
Zhang Jiangli,
Albert Andreas,
Winkler Barbro,
Lang Hans,
Buegger Franz,
Gaupels Frank,
Heller Werner,
Michalke Bernhard,
Sarioglu Hakan,
Schnitzler JörgPeter,
Hebelstrup Kim Henrik,
Durner Jörg,
Lindermayr Christian
Publication year - 2017
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12773
Subject(s) - arabidopsis , nitrogen fixation , biology , nitric oxide , arabidopsis thaliana , symbiosis , botany , context (archaeology) , photosynthesis , shoot , horticulture , mutant , chemistry , nitrogen , biochemistry , gene , bacteria , paleontology , genetics , endocrinology , organic chemistry
Nitric oxide (NO) is an important signalling molecule that is involved in many different physiological processes in plants. Here, we report about a NO‐fixing mechanism in Arabidopsis , which allows the fixation of atmospheric NO into nitrogen metabolism. We fumigated Arabidopsis plants cultivated in soil or as hydroponic cultures during the whole growing period with up to 3 ppmv of NO gas. Transcriptomic, proteomic and metabolomic analyses were used to identify non‐symbiotic haemoglobin proteins as key components of the NO‐fixing process. Overexpressing non‐symbiotic haemoglobin 1 or 2 genes resulted in fourfold higher nitrate levels in these plants compared with NO‐treated wild‐type. Correspondingly, rosettes size and weight, vegetative shoot thickness and seed yield were 25, 40, 30, and 50% higher, respectively, than in wild‐type plants. Fumigation with 250 ppbv 15 NO confirmed the importance of non‐symbiotic haemoglobin 1 and 2 for the NO‐fixation pathway, and we calculated a daily uptake for non‐symbiotic haemoglobin 2 overexpressing plants of 250 mg N/kg dry weight. This mechanism is probably important under conditions with limited N supply via the soil. Moreover, the plant‐based NO uptake lowers the concentration of insanitary atmospheric NOx, and in this context, NO‐fixation can be beneficial to air quality.