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Diacylglycerol kinases activate tobacco NADPH oxidase‐dependent oxidative burst in response to cryptogein
Author(s) -
Cacas JeanLuc,
GerbeauPissot Patricia,
Fromentin Jérôme,
Cantrel Catherine,
Thomas Dominique,
Jeannette Emmanuelle,
Kalachova Tetiana,
Mongrand Sébastien,
SimonPlas Françoise,
Ruelland Eric
Publication year - 2017
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12771
Subject(s) - diacylglycerol kinase , respiratory burst , nadph oxidase , biochemistry , nicotinamide adenine dinucleotide phosphate , biology , microbiology and biotechnology , elicitor , phospholipase d , phosphatidic acid , kinase , chemistry , oxidase test , signal transduction , protein kinase c , reactive oxygen species , enzyme , phospholipid , membrane
Cryptogein is a 10 kDa protein secreted by the oomycete Phytophthora cryptogea that activates defence mechanisms in tobacco plants. Among early signalling events triggered by this microbial‐associated molecular pattern is a transient apoplastic oxidative burst which is dependent on the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity of the RESPIRATORY BURST OXIDASE HOMOLOG isoform D (RBOHD). Using radioactive [ 33 P]‐orthophosphate labelling of tobacco Bright Yellow‐2 suspension cells, we here provide in vivo evidence for a rapid accumulation of phosphatidic acid (PA) in response to cryptogein because of the coordinated onset of phosphoinositide‐dependent phospholipase C and diacylglycerol kinase (DGK) activities. Both enzyme specific inhibitors and silencing of the phylogenetic cluster III of the tobacco DGK family were found to reduce PA production upon elicitation and to strongly decrease the RBOHD‐mediated oxidative burst. Therefore, it appears that PA originating from DGK controls NADPH‐oxidase activity. Amongst cluster III DGKs, the expression of DGK5‐like was up‐regulated in response to cryptogein. Besides DGK5‐like is likely to be the main cluster III DGK isoform silenced in one of our mutant lines, making it a strong candidate for the observed response to cryptogein. The relevance of these results is discussed with regard to early signalling lipid‐mediated events in plant immunity.