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Signal transduction and regulation of IbpreproHypSys in sweet potato
Author(s) -
Li YuChi,
Wan WeiLin,
Lin JengShane,
Kuo YunWei,
King YuChi,
Chen YuChi,
Jeng ShihTong
Publication year - 2016
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12729
Subject(s) - transduction (biophysics) , signal transduction , biology , botany , microbiology and biotechnology
Abstract Hydroxyproline‐rich glycopeptides (HypSys) are small signalling peptides containing 18–20 amino acids. The expression of IbpreproHypSys , encoding the precursor of Ib HypSys, was induced in sweet potato ( Ipomoea batatas cv. Tainung 57) through wounding and Ib HypSys treatments by using jasmonate and H 2 O 2 . Transgenic sweet potatoes overexpressing (OE) and silencing [RNA interference (RNAi)] IbpreproHypSys were created. The expression of the wound‐inducible gene for ipomoelin ( IPO ) in the local and systemic leaves of OE plants was stronger than the expression in wild‐type (WT) and RNAi plants after wounding. Furthermore, grafting experiments indicated that IPO expression was considerably higher in WT stocks receiving wounding signals from OE than from RNAi scions. However, wounding WT scions highly induced IPO expression in OE stocks. These results indicated that IbpreproHypSys expression contributed towards sending and receiving the systemic signals that induced IPO expression. Analysing the genes involved in the phenylpropanoid pathway demonstrated that lignin biosynthesis was activated after synthetic Ib HypSys treatment. IbpreproHypSys expression in sweet potato suppressed Spodoptera litura growth . In conclusion, wounding induced the expression of IbpreproHypSys , whose protein product was processed into Ib HypSys. Ib HypSys stimulated IbpreproHypSys and IPO expression and enhanced lignin biosynthesis, thus protecting plants from insects.