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Biogenesis of water splitting by photosystem II during de‐etiolation of barley ( Hordeum vulgare L.)
Author(s) -
Shevela Dmitriy,
Arnold Janine,
Reisinger Veronika,
Berends HansMartin,
Kmiec Karol,
Koroidov Sergey,
Bue Ann Kristin,
Messinger Johannes,
Eichacker Lutz A.
Publication year - 2016
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12719
Subject(s) - photosystem ii , thylakoid , hordeum vulgare , etiolation , chloroplast , protochlorophyllide , biology , photosystem , biophysics , photosystem i , photosynthesis , photochemistry , botany , chemistry , biochemistry , poaceae , gene , enzyme
Etioplasts lack thylakoid membranes and photosystem complexes. Light triggers differentiation of etioplasts into mature chloroplasts, and photosystem complexes assemble in parallel with thylakoid membrane development. Plastids isolated at various time points of de‐etiolation are ideal to study the kinetic biogenesis of photosystem complexes during chloroplast development. Here, we investigated the chronology of photosystem II (PSII) biogenesis by monitoring assembly status of chlorophyll‐binding protein complexes and development of water splitting via O 2 production in plastids (etiochloroplasts) isolated during de‐etiolation of barley ( Hordeum vulgare L.). Assembly of PSII monomers, dimers and complexes binding outer light‐harvesting antenna [PSII‐light‐harvesting complex II (LHCII) supercomplexes] was identified after 1, 2 and 4 h of de‐etiolation, respectively. Water splitting was detected in parallel with assembly of PSII monomers, and its development correlated with an increase of bound Mn in the samples. After 4 h of de‐etiolation, etiochloroplasts revealed the same water‐splitting efficiency as mature chloroplasts. We conclude that the capability of PSII to split water during de‐etiolation precedes assembly of the PSII‐LHCII supercomplexes. Taken together, data show a rapid establishment of water‐splitting activity during etioplast‐to‐chloroplast transition and emphasize that assembly of the functional water‐splitting site of PSII is not the rate‐limiting step in the formation of photoactive thylakoid membranes.