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miRNA‐mediated auxin signalling repression during Vat ‐mediated aphid resistance in Cucumis melo
Author(s) -
Sattar Sampurna,
AddoQuaye Charles,
Thompson Gary A.
Publication year - 2016
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12645
Subject(s) - auxin , aphis gossypii , biology , cucumis , melon , microrna , microbiology and biotechnology , psychological repression , aphid , transcription factor , genetics , botany , gene , gene expression , horticulture , aphididae , pest analysis , homoptera
Resistance to Aphis gossypii in melon is attributed to the presence of the single dominant R gene virus aphid transmission ( Vat) , which is biologically expressed as antibiosis, antixenosis and tolerance. However, the mechanism of resistance is poorly understood at the molecular level. Aphid‐induced transcriptional changes, including differentially expressed miRNA profiles that correspond to resistance interaction have been reported in melon. The potential regulatory roles of miRNAs in Vat‐ mediated aphid resistance were further revealed by identifying the specific miRNA degradation targets. A total of 70 miRNA:target pairs, including 28 novel miRNA:target pairs, for the differentially expressed miRNAs were identified: 11 were associated with phytohormone regulation, including six miRNAs that potentially regulate auxin interactions. A model for a redundant regulatory system of miRNA‐mediated auxin insensitivity is proposed that incorporates auxin perception, auxin modification and auxin‐regulated transcription. Chemically inhibiting the transport inhibitor response‐1 (TIR‐1) auxin receptor in susceptible melon tissues provides in vivo support for the model of auxin‐mediated impacts on A. gossypii resistance.