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Deriving C 4 photosynthetic parameters from combined gas exchange and chlorophyll fluorescence using an Excel tool: theory and practice
Author(s) -
Bellasio Chandra,
Beerling David J,
Griffiths Howard
Publication year - 2016
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12626
Subject(s) - photorespiration , photosynthesis , biome , chlorophyll fluorescence , biological system , computer science , interpretation (philosophy) , stomatal conductance , chemistry , biochemical engineering , botany , biology , ecology , ecosystem , engineering , programming language
The higher photosynthetic potential of C 4 plants has led to extensive research over the past 50 years, including C 4 ‐dominated natural biomes, crops such as maize, or for evaluating the transfer of C 4 traits into C 3 lineages. Photosynthetic gas exchange can be measured in air or in a 2% Oxygen mixture using readily available commercial gas exchange and modulated PSII fluorescence systems. Interpretation of these data, however, requires an understanding (or the development) of various modelling approaches, which limit the use by non‐specialists. In this paper we present an accessible summary of the theory behind the analysis and derivation of C 4 photosynthetic parameters, and provide a freely available Excel Fitting Tool (EFT), making rigorous C 4 data analysis accessible to a broader audience. Outputs include those defining C 4 photochemical and biochemical efficiency, the rate of photorespiration, bundle sheath conductance to CO 2 diffusion and the in vivo biochemical constants for PEP carboxylase. The EFT compares several methodological variants proposed by different investigators, allowing users to choose the level of complexity required to interpret data. We provide a complete analysis of gas exchange data on maize (as a model C 4 organism and key global crop) to illustrate the approaches, their analysis and interpretation. © 2015 John Wiley & Sons Ltd