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New prenyllipid metabolites identified in A rabidopsis during photo‐oxidative stress
Author(s) -
Dłużewska Jolanta,
Zieliński Kamil,
Nowicka Beatrycze,
Szymańska Renata,
Kruk Jerzy
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12580
Subject(s) - plastoquinone , arabidopsis , singlet oxygen , photosystem ii , arabidopsis thaliana , mutant , biology , chloroplast , thylakoid , wild type , biochemistry , photosynthesis , botany , chemistry , biophysics , gene , oxygen , organic chemistry
In the present study, we have identified new prenyllipid metabolites formed during high light stress in A rabidopsis thaliana , whose origin and function remained unknown so far. It was found that plastoquinone‐ C accumulates mainly in the reduced form under high light conditions, as well as during short‐term excess light illumination both in the wild‐type and tocopherol biosynthetic vte1 mutant, suggesting that plastoquinone‐ C , a singlet oxygen‐derived prenyllipid, is reduced in chloroplasts by photosystem II or enzymatically, outside thylakoids. Plastoquinone‐ B , a fatty acid ester of plastoquinone‐ C , was identified for the first time in Arabidopsis in high light grown wild‐type plants and during short‐time, excess light illumination of the wild‐type plants and the vte1 mutant. The gene expression analysis showed that vte2 gene is most pronouncedly up‐regulated among the prenyllipid biosynthetic genes under high light and induction of its expression is mainly caused by an increased level of singlet oxygen, as was demonstrated in experiments with D 2 O ‐treated plants under excess light conditions.