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A rabidopsis thaliana CML 25 mediates the Ca 2+ regulation of K + transmembrane trafficking during pollen germination and tube elongation
Author(s) -
Wang ShuangShuang,
Diao WenZhu,
Yang Xue,
Qiao Zhu,
Wang Mei,
Acharya Biswa R.,
Zhang Wei
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12559
Subject(s) - pollen , pollen tube , germination , mutant , elongation , biology , botany , biophysics , biochemistry , gene , materials science , pollination , ultimate tensile strength , metallurgy
The concentration alteration of cytosolic‐free calcium ([ Ca 2+ ] cyt ) is a well‐known secondary messenger in plants and plays important roles during pollen grain germination and tube elongation. Here we demonstrate that CML 25, a member of calmodulin‐like proteins, has Ca 2+ ‐binding activity and plays a role in pollen grain germination, tube elongation and seed setting. CML 25 transcript was abundant in mature pollen grains and pollen tubes, and its product CML 25 protein was primarily directed to the cytoplasm. Two independent CML 25 loss‐of‐function T‐DNA insertion mutants suffered a major reduction in both the rate of pollen germination and the elongation of the pollen tube. Also, pollen grains of cml25 mutants were less sensitive to the external K + and Ca 2+ concentration than wild‐type pollen. The disruption of CML 25 increased the [ Ca 2+ ] cyt in both the pollen grain and the pollen tube, which in turn impaired the Ca 2+ ‐dependent inhibition of whole‐cell inward K + currents in protoplasts prepared from these materials (pollen grain and pollen tube). Complementation of cml25‐1 mutant resulted in the recovery of wild‐type phenotype. Our findings indicate that CML 25 is an important transducer in the Ca 2+ ‐mediated regulation of K + influx during pollen germination and tube elongation.