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H + ‐pyrophosphatase from S alicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in A rabidopsis and wheat
Author(s) -
Lv Sulian,
Jiang Ping,
Nie Lingling,
Chen Xianyang,
Tai Fang,
Wang Duoliya,
Fan Pengxiang,
Feng Juanjuan,
Bao Hexigeduleng,
Wang Jinhui,
Li Yinxin
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12557
Subject(s) - arabidopsis , shoot , chemistry , halophyte , salinity , genetically modified crops , sugar beet , starch , botany , nitrate , biochemistry , biology , transgene , horticulture , gene , mutant , ecology , organic chemistry
High salinity and nitrogen ( N ) deficiency in soil are two key factors limiting crop productivity, and they usually occur simultaneously. Here we firstly found that H + ‐ PP ase is involved in salt‐stimulated NO 3 − uptake in the euhalophyte S alicornia europaea . Then, two genes (named SeVP1 and SeVP2 ) encoding H + ‐ PP ase from S . europaea were characterized. The expression of SeVP1 and SeVP2 was induced by salt stress and N starvation. Both SeVP1 or SeVP2 transgenic A rabidopsis and wheat plants outperformed the wild types ( WTs ) when high salt and low N occur simultaneously. The transgenic A rabidopsis plants maintained higher K + / Na + ratio in leaves and exhibited increased NO 3 − uptake, inorganic pyrophosphate‐dependent vacuolar nitrate efflux and assimilation capacity under this double stresses. Furthermore, they had more soluble sugars in shoots and roots and less starch accumulation in shoots than WT . These performances can be explained by the up‐regulated expression of ion, nitrate and sugar transporter genes in transgenic plants. Taken together, our results suggest that up‐regulation of H + ‐ PP ase favours the transport of photosynthates to root, which could promote root growth and integrate N and carbon metabolism in plant. This work provides potential strategies for improving crop yields challenged by increasing soil salinization and shrinking farmland.