Premium
Rice PCR1 influences grain weight and Z n accumulation in grains
Author(s) -
Song WonYong,
Lee HyunSook,
Jin SangRak,
Ko Donghwi,
Martinoia Enrico,
Lee Youngsook,
An Gynheung,
Ahn SangNag
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12553
Subject(s) - wild type , oryza sativa , arabidopsis , mutant , biology , arabidopsis thaliana , yeast , botany , microbiology and biotechnology , biochemistry , gene
Proteins containing a placenta‐specific 8 domain ( PLAC8 ) function as major organ size regulators in S olanum lycopersicum and Z ea may , and putative metal ion transporters in A rabidopsis thaliana , O ryza sativa and B rassica juncea . However, it is unknown how PLAC8 domain‐containing proteins fulfill such diverse roles. Here, we found that plant cadmium resistance 1 ( PCR1 ) influences both zinc ( Z n) accumulation and grain weight in rice. OsPCR1 knockout and knockdown lines produced lighter grains than the wild type, while OsPCR1 overexpression lines produced heavier grains. Furthermore, the grains of OsPCR1 knockdown lines exhibited substantially higher Z n and lower cadmium ( C d) concentrations than the control, as did yeast heterologously expressing OsPCR1 . Through sequence analysis, we showed that the amino acid sequence of japonica‐type PCR1 was distinct from that of indica‐type and wild rice accessions. This difference was correlated with distinct Z n‐related phenotypes. Japonica‐type PCR1 had a shorter N ‐terminus than did PCR1 in the other rice types, and yeast heterologously expressing japonica‐type PCR1 was more sensitive to Z n than was yeast expressing indica‐type PCR1 . Furthermore, japonica‐type grains accumulated less Z n than did indica‐type grains. Our study suggests that rice PCR1 maintains metal ion homeostasis and grain weight and might have been selected for during domestication.