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L accaria bicolor aquaporin LbAQP1 is required for H artig net development in trembling aspen ( P opulus tremuloides )
Author(s) -
NavarroRóDenas Alfonso,
Xu Hao,
Kemppainen Minna,
Pardo Alejandro G.,
Zwiazek Janusz J.
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12552
Subject(s) - mycelium , hypha , yeast , aquaporin , botany , biology , microbiology and biotechnology , chemistry , biochemistry
Abstract The development of ectomycorrhizal associations is crucial for growth of many forest trees. However, the signals that are exchanged between the fungus and the host plant during the colonization process are still poorly understood. In this study, we have identified the relationship between expression patterns of L accaria bicolor aquaporin LbAQP1 and the development of ectomycorrhizal structures in trembling aspen ( P opulus tremuloides ) seedlings. The peak expression of LbAQP1 was 700‐fold higher in the hyphae within the root than in the free‐living mycelium after 24 h of direct interaction with the roots. Moreover, in LbAQP1 knock‐down strains, a non‐mycorrhizal phenotype was developed without the H artig net and the expression of the mycorrhizal effector protein MiSSP7 quickly declined after an initial peak on day 5 of interaction of the fungal hyphae with the roots. The increase in the expression of LbAQP1 required a direct contact of the fungus with the root and it modulated the expression of MiSSP7 . We have also determined that LbAQP1 facilitated NO , H 2 O 2 and CO 2 transport when heterologously expressed in yeast. The report demonstrates that the L . bicolor aquaporin LbAQP1 acts as a molecular signalling channel, which is fundamental for the development of H artig net in root tips of P . tremuloides .