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Rice cyclophilin OsCYP18‐2 is translocated to the nucleus by an interaction with SKIP and enhances drought tolerance in rice and Arabidopsis
Author(s) -
Lee Sang Sook,
Park Hyun Ji,
Yoon Dae Hwa,
Kim BeomGi,
Ahn Jun Cheul,
Luan Sheng,
Cho Hye Sun
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12531
Subject(s) - cyclophilin , arabidopsis , biology , arabidopsis thaliana , gene , microbiology and biotechnology , peptidylprolyl isomerase , rna splicing , caenorhabditis elegans , prolyl isomerase , genetics , schizosaccharomyces pombe , saccharomyces cerevisiae , isomerase , mutant , pin1 , rna
Cyclophilin 18‐2 ( CYP18‐2 ) genes, homologues of human peptidyl‐prolyl isomerase‐like 1 ( PPiL1 ), are conserved across multicellular organisms and S chizosaccharomyces pombe . Although PPiL1 is known to interact with ski‐interacting protein ( SKIP ), a transcriptional co‐regulator and spliceosomal component, there have been no functional analyses of PPiL1 homologues in plants. Rice cyclophilin 18‐2 ( OsCYP 18‐2) bound directly to amino acids 56–95 of OsSKIP and its binding was independent of cyclosporin A , a cyclophilin‐binding drug. Moreover, OsCYP 18‐2 exhibited PPIase activity regardless of its interaction with OsSKIP . Therefore, the binding site for OsCYP 18‐2's interaction with SKIP was distinct from the PPIase active site. OsCYP 18‐2's interaction with SKIP full‐length protein enabled OsCYP 18‐2's translocation from the cytoplasm into the nucleus and AtSKIP interacted in planta with both AtCYP 18‐2 and OsCYP 18‐2. Drought and salt stress induced similar expression of OsCYP 18‐2 and OsSKIP . Overexpression of OsCYP 18‐2 in transgenic rice and A rabidopsis thaliana plants enhanced drought tolerance and altered expression and pre‐ mRNA splicing patterns of stress‐related genes in Arabidopsis under drought conditions. Furthermore, OsCYP 18‐2 caused transcriptional activation with/without OsSKIP in the GAL 4 system of yeast; thus the OsSKIP ‐ OsCYP 18‐2 interaction has an important role in the transcriptional and post‐transcriptional regulation of stress‐related genes and increases tolerance to drought stress.