Premium
Breakdown of middle lamella pectin by ● OH during rapid abscission in A zolla
Author(s) -
YAMADA YOSHIYA,
KOIBUCHI MIZUKI,
MIYAMOTO KENSUKE,
UEDA JUNICHI,
UHEDA EIJI
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12505
Subject(s) - abscission , middle lamella , lamella (surface anatomy) , botany , petiole (insect anatomy) , cell wall , biology , chemistry , anatomy , hymenoptera
A zolla , a small water fern, abscises its roots and branches within 30 min upon treatment with various stresses. This study was conducted to test whether, in the rapid abscission that occurs in A zolla , breakdown of wall components of abscission zone cells by ● OH is involved. Experimentally generated ● OH caused the rapid separation of abscission zone cells from detached roots and the rapid shedding of roots from whole plants. Electron microscopic observations revealed that ● OH rapidly and selectively dissolved a well‐developed middle lamella between abscission zone cells and resultantly caused rapid cell separation and shedding. Treatment of abscission zones of I mpatiens leaf petiole with ● OH also accelerated the separation of abscission zone cells. However, compared with that of A zolla roots, accelerative effects in I mpatiens were weak. A large amount of ● OH was cytochemically detected in abscission zone cells both of A zolla roots and of I mpatiens leaf petioles. These results suggest that ● OH is involved in the cell separation process not only in the rapid abscission in A zolla but also in the abscission of I mpatiens . However, for rapid abscission to occur, a well‐developed middle lamella, a unique structure, which is sensitive to the attack of ● OH , might be needed.